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UID:1280@biotech.technion.ac.il
DTSTART;TZID=Asia/Jerusalem:20261125T140000
DTEND;TZID=Asia/Jerusalem:20261125T150000
DTSTAMP:20261025T133901Z
URL:https://biotech.technion.ac.il/events/%d7%a1%d7%9e%d7%99%d7%a0%d7%a8-%
 d7%90%d7%95%d7%a8%d7%97-%d7%93%d7%a8-%d7%9c%d7%99%d7%90%d7%95%d7%a8-%d7%9c
 %d7%95%d7%91%d7%9c-2/
SUMMARY:סמינר אורח: ד"ר ליאור לובל
DESCRIPTION:Beyond who is there: a post-translationallayer in the diet–mi
 crobiome relationship\nAbstract:  ** Lecture will be given in English**\n
 The gut microbiome’s response to diet is usually read as achange in comm
 unity composition — which organisms are present and which genesthey carr
 y. Whether dietary substrate also reaches the microbial proteomepost-trans
 lationally is largely unexplored. Fermentation of soluble fiberyields acet
 ate and acetyl-phosphate\, the donors for bacterial lysineacetylation\, gi
 ving a direct chemical route from diet to protein modification.\nWe fed mi
 ce soluble (pectin and inulin) or insoluble (cellulose)fiber for four week
 s and enriched acetylated proteins from cecal and fecalcontents by anti-ac
 etyllysine immunoprecipitation at the intact-protein level\,analyzing both
  the captured fraction and its matched flow-through bydata-dependent mass 
 spectrometry against a sample-matched\, metagenome-derivedprotein database
 .\nSoluble fiber raised cecal acetate and broadenedacetylated-protein capt
 ure roughly six-fold\, with no overlap between dietgroups\, more proteins 
 captured rather than more of each\, while matched bulkflow-through breadth
  was unchanged and pre-diet samples showed no asymmetry.The captured set w
 as over-represented in fermentation and nitrogen-handlingenzymes. Glutamat
 e dehydrogenase was retained in the cecal acetylated fractionwhile its bul
 k abundance there fell and both the gene and its carrier lineagesdeclined 
 in the fecal metagenome\, dissociating post-translational state fromabunda
 nce and gene dosage.\nI will discuss what this designcan and cannot establ
 ish\, including a direct cecal ammonia measurement thatbehaves as dilution
  rather than reduced production\, and the recombinantenzymology needed to 
 test whether this acetylation is regulatory.
ATTACH;FMTTYPE=image/jpeg:https://biotech.technion.ac.il/wp-content/upload
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CATEGORIES:סמינרים
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TZID:Asia/Jerusalem
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