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TZID:Asia/Jerusalem
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UID:1160@biotech.technion.ac.il
DTSTART;TZID=Asia/Jerusalem:20260520T140000
DTEND;TZID=Asia/Jerusalem:20260520T150000
DTSTAMP:20260506T053747Z
URL:https://biotech.technion.ac.il/events/%d7%a1%d7%9e%d7%99%d7%a0%d7%a8-%
 d7%90%d7%95%d7%a8%d7%97-%d7%93%d7%a8-%d7%a2%d7%95%d7%a4%d7%a8%d7%99-%d7%9c
 %d7%95%d7%99/
SUMMARY:סמינר אורח: ד"ר עופרי לוי
DESCRIPTION:Functional Genomics Resolves the Molecular Architecture of Anti
 fungal Tolerance and Plant-Derived Synergy\n ** Lecture will be given in 
 English**\nAbstract:\nInvasive fungal infections and escalating resistance
  severely compromise clinical efficacy\, necessitating the discovery of no
 vel therapeutic targets and agents. To comprehensively map functional vuln
 erabilities and discover synergistic compounds\, we integrated high-throug
 hput plant extract screening with genome-wide CRISPR interference (CRISPRi
 ) profiling .\nUnder azole-induced stress in Saccharomyces cerevisiae\, ou
 r screen defined distinct architectural modules governing survival. We dem
 onstrate that adaptive fluconazole tolerance is driven by translational at
 tenuation. Targeted repression of ribosome biogenesis components\, notably
  RPS14A and RRB1\, confers massive fitness advantages .\nParallel screenin
 g of 794 plant extracts identified tea seed and Gentiana lutea as potent i
 nhibitors of Candida albicans proliferation\, reducing growth by &gt\;40%.
  Functional interrogation of the plant phenolic ferulic acid (FA) linked i
 ts mechanism to ergosterol biosynthesis perturbation. Specifically\, FA pr
 essure upregulates HMG1 and HMG2\, while ERG9 repression confers marked FA
  resistance. Crucially\, FA exhibits potent synergy with fluconazole\, red
 ucing its minimal inhibitory concentration by approximately 8-fold in C. a
 lbicans and restoring susceptibility in azole-resistant strains. Furthermo
 re\, FA demonstrated dose-dependent lesion suppression in a Cochliobolus h
 eterostrophus maize infection model .\nCollectively\, this combinatorial f
 unctional genomics approach delineates the molecular landscape of drug tol
 erance and validates plant-derived modulators as next-generation synergist
 ic antifungal interventions.
CATEGORIES:סמינרים
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