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UID:1211@biotech.technion.ac.il
DTSTART;TZID=Asia/Jerusalem:20260722T110000
DTEND;TZID=Asia/Jerusalem:20260722T120000
DTSTAMP:20260715T130210Z
URL:https://biotech.technion.ac.il/events/%d7%a1%d7%9e%d7%99%d7%a0%d7%a8-%
 d7%a1%d7%99%d7%95%d7%9d-%d7%93%d7%95%d7%a7%d7%98%d7%95%d7%a8%d7%98-%d7%94%
 d7%95%d7%a0%d7%92%d7%a9%d7%99%d7%90%d7%a0%d7%92-%d7%9c%d7%99%d7%95-%d7%9e%
 d7%94%d7%9e%d7%a2/
SUMMARY:סמינר סיום דוקטורט: הונגשיאנג ליו\, מ
 המעבדה של פרופ' דגנית דנינו ופרופ' הרולד 
 קורק
DESCRIPTION:Research Topic: Porous starch from dual-amylase hydrolysis: str
 ucture\, regulation and functional application\nAbstract: ** Lecture will 
 be given in English**\nStarch is a highly abundant biopolymer whose native
  granules vary across botanical origins. Porous starch is fabricated by ge
 nerating interconnected pores while preserving granular integrity\; subgel
 atinization amylolysis serves as an efficient preparation route.\nWe inves
 tigated evolutions in morphology\, porosity\, and physicochemical properti
 es during synergistic dual-enzyme (α-amylase and γ-amylase) modification
  of three maize starches. Random endo-cleavage by α-amylase yields abunda
 nt non-reducing ends\, accelerating inward hydrolysis by exo-acting γ-amy
 lase within the alternating amorphous–crystalline lamellae. To regulate 
 this reaction\, we proposed the Granular Starch Hydrolysis Unit (GSHU)\, a
  flexible parameter that reflects reducing sugar release and quantifies st
 arch susceptibility to enzymatic attack. Benefiting from uniformly distrib
 uted branch points and inherent surface nanopores\, A-type starches reache
 d optimal porosity under enzyme dosages predicted by GSHU. Importantly\, t
 his predictive performance remained effective even after high-pressure pre
 treatment (400 MPa\, 10 min). To resolve internal architectures\, epoxy-re
 sin-embedded granules were cut into 100-nm ultra-thin sections for cross-s
 ectional SEM observation. Focused ion beam (FIB) milling allowed sequentia
 l imaging at 100/200 nm intervals\, and the serial micrographs were recons
 tructed into three-dimensional models using Amira software. Finally\, curc
 umin-loaded prolamin nanoparticles were successfully incorporated into por
 ous starch\, greatly enhancing the stability and bioaccessibility of this 
 hydrophobic polyphenol.\nOverall\, this work provides key insights into en
 zymatic starch modification and lays a solid foundation for the design of 
 advanced functional starch-based materials.
CATEGORIES:סמינרים
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