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UID:1185@biotech.technion.ac.il
DTSTART;TZID=Asia/Jerusalem:20260701T140000
DTEND;TZID=Asia/Jerusalem:20260701T150000
DTSTAMP:20260621T092045Z
URL:https://biotech.technion.ac.il/events/%d7%a1%d7%9e%d7%99%d7%a0%d7%a8-%
 d7%a1%d7%99%d7%95%d7%9d-%d7%93%d7%95%d7%a7%d7%98%d7%95%d7%a8%d7%98-%d7%9b%
 d7%99%d7%90%d7%9f-%d7%a2%d7%95%d7%90%d7%95%d7%93%d7%94-%d7%9e%d7%94%d7%9e%
 d7%a2%d7%91%d7%93/
SUMMARY:סמינר סיום דוקטורט: כיאן עואודה\, מהמ
 עבדה של פרופ' אסתי סגל
DESCRIPTION:Development of a Porous Silicon-Based Optical Biosensor for Mul
 tiplex Detection of Biomarkers Integrated with a 3D-Printed Microfluidic S
 ystem\nAbstract: ** Lecture will be given in English**\nPorous silicon (PS
 i)-based optical biosensors are attractive platforms for label-free biomar
 ker detection because of their large internal surface area\, tunable nanos
 tructure\, and compatibility with optical transduction. However\, the broa
 der implementation of PSi biosensors in real-world applications is often l
 imited by two major challenges: hindered mass transport within the porous 
 matrix and biofouling caused by nonspecific adsorption in complex biologic
 al media. These challenges reduce sensitivity\, selectivity\, reproducibil
 ity\, and dynamic range\, particularly when detecting low-abundance biomar
 kers in clinically relevant samples.\nThis work addresses these limitation
 s using a PSi Fabry–Pérot aptasensor platform for lactoferrin (LF)\, a 
 biomarker associated with gastrointestinal (GI) inflammation. First\, we h
 ave rationally optimized the porous nanostructure to improve analyte trans
 port to and withing the sensor. Integration with microfluidic mixing devic
 es enhanced convection and LF delivery to the sensor interface and signifi
 cantly improved sensitivity\, reducing the limit of detection from 50 nM u
 nder diffusion-dominated conditions to 3 nM under active mixing. The secon
 d part of this research focused on improving the biosensor performance in 
 complex GI fluids. Conventional PEG passivation was insufficient to preven
 t biofouling\, leading to a marked loss of sensitivity. To overcome this l
 imit\, zwitterionic E/K-rich peptides were covalently immobilized onto the
  PSi surface. These coatings provided superior resistance to nonspecific a
 dsorption and restored the aptasensor performance in GI fluid\, improving 
 LF limit of detection from 600 nM to 49 nM\, comparable to the response ac
 hieved in buffer solution.\nFinally\, robotic microspotting was employed t
 o create a multiplexed sensing platform through spatially resolved immobil
 ization of LF-specific aptamers and aptamers for additional targets. This 
 generated spatially resolved sensing domains and demonstrated the feasibil
 ity of multiplexed detection on a single PSi chip. Collectively\, these ad
 vances establish a scalable PSi-based lab-on-chip platform for sensitive a
 nd multiplexed biomarker detection in complex biological samples.
CATEGORIES:סמינרים
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