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UID:1199@biotech.technion.ac.il
DTSTART;TZID=Asia/Jerusalem:20260729T140000
DTEND;TZID=Asia/Jerusalem:20260729T150000
DTSTAMP:20260617T125502Z
URL:https://biotech.technion.ac.il/events/%d7%a1%d7%9e%d7%99%d7%a0%d7%a8-%
 d7%a1%d7%99%d7%95%d7%9d-%d7%93%d7%95%d7%a7%d7%98%d7%95%d7%a8%d7%98-%d7%a1%
 d7%aa%d7%99%d7%95-%d7%a4%d7%9c%d7%93-%d7%9e%d7%94%d7%9e%d7%a2%d7%91%d7%93%
 d7%94-%d7%a9%d7%9c/
SUMMARY:סמינר סיום דוקטורט: סתיו פלד\, מהמעבד
 ה של פרופ' יואב ליבני
DESCRIPTION:Oligosaccharides-protein conjugates for selective targeting of 
 proteins to probiotic bacteria in the colon\nAbstract: ** Lecture will be 
 given in English**\nThe human gut microbiome plays a central role in host 
 health through its extensive interactions with host metabolism\, immune fu
 nction\, and intestinal barrier integrity. Prebiotics are widely used to b
 eneficially modulate the gut microbiota\; however\, currently available pr
 ebiotics are predominantly carbohydrate-based and do not address the limit
 ed availability of protein in the colon. Since approximately 90% of dietar
 y proteins are digested and absorbed in the upper gastrointestinal tract\,
  colonic microorganisms compete for residual unabsorbed peptides reaching 
 the colon.\nThis research aimed to develop a next generation prebiotic pla
 tform: protein-containing prebiotics\, designed to selectively deliver bot
 h prebiotic carbohydrates and protein substrate to gut probiotics\, thereb
 y enhancing their growth and beneficial metabolic activity relative to con
 ventional carbohydrate-only prebiotics. To achieve this\, Maillard conjuga
 tes composed of the prebiotic human milk oligosaccharide 2′-fucosyllacto
 se (2′-FL) and protein peptic-then-tryptic hydrolysates were developed\,
  forming self-assembled micellar structures with a protein core and an oli
 gosaccharide shell. This structure retards upper gastrointestinal protein 
 absorption\, while aiming to enable oligosaccharide-mediated\, selectively
  targeted delivery to colonic probiotics. The first proof-of-concept syste
 m\, based on lactoferrin hydrolysates\, significantly increased the abunda
 nce of short-chain fatty acid (SCFAs)-producing bacterial taxa\, enhanced 
 colonic SCFAs levels\, and reduced plasma lipopolysaccharide levels in mic
 e\, demonstrating superior prebiotic efficacy and gut-barrier integrity pr
 omotion compared with unconjugated components and saline controls.\nTo imp
 rove scalability and sustainability\, potato protein hydrolysates were sub
 sequently evaluated as an alternative protein source. The resulting conjug
 ates promoted health-associated bacterial taxa\, enhanced microbial SCFAs 
 production\, and improved markers of gut barrier integrity and metabolic h
 ealth in-vivo\, outperforming conventional prebiotic and saline controls. 
 These findings validate potato protein hydrolysates as a cost-effective\, 
 non-allergenic and sustainable alternative to lactoferrin hydrolysates. Im
 portantly\, no increase in harmful proteolytic fermentation byproducts was
  observed in either system. Finally\, the platform was expanded into a mul
 tifunctional colonic delivery system\, through the incorporation of the pr
 ebiotic polyphenol resveratrol into the conjugate assemblies\, and their s
 ubsequent stabilization by genipin-mediated crosslinking. Crosslinking red
 uced proteolytic susceptibility and premature resveratrol release\, suppor
 ting the feasibility of co-delivering prebiotic carbohydrates\, peptides\,
  and polyphenols to the colon within a single delivery vehicle\, offering 
 a novel strategy for enhanced beneficial modulation of the gut microbiota 
 and promotion of gut and metabolic health.\nCollectively\, this work estab
 lishes protein-containing prebiotics as a novel strategy for targeted micr
 obiome modulation and provides a versatile platform for the development of
  next-generation functional foods and gut- and metabolic-health interventi
 ons.
CATEGORIES:סמינרים
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TZID:Asia/Jerusalem
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DTSTART:20260327T030000
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