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TZID:Asia/Jerusalem
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UID:1222@biotech.technion.ac.il
DTSTART;TZID=Asia/Jerusalem:20261005T140000
DTEND;TZID=Asia/Jerusalem:20261005T143000
DTSTAMP:20260915T061737Z
URL:https://biotech.technion.ac.il/events/%d7%a1%d7%9e%d7%99%d7%a0%d7%a8-%
 d7%a1%d7%99%d7%95%d7%9d-%d7%9e%d7%92%d7%99%d7%a1%d7%98%d7%a8-%d7%92%d7%9c%
 d7%99-%d7%a8%d7%95%d7%9f-%d7%9e%d7%94%d7%9e%d7%a2%d7%91%d7%93%d7%94-%d7%a9
 %d7%9c-%d7%a4/
SUMMARY:סמינר סיום מגיסטר: גלי רון\, מהמעבדה ש
 ל פרופ' אסתי סגל
DESCRIPTION:Research Topic: Photonic Micro-Patterned Silicon Chip for Selec
 tive Bacterial Spatial Colonization\nAbstract: ** Lecture will be given in
  English**\nBacterial adhesion to solid surfaces is governed by a complex 
 interplay between bacterial characteristics\, environmental conditions\, a
 nd surface physicochemical properties. Understanding these interactions is
  important for elucidating early bacterial colonization and for the ration
 al design of surfaces that can modulate bacterial attachment and spatial o
 rganization.\nIn this work\, we developed a platform that integrates a sil
 icon photonic chip\, with a well-defined micropore geometry\, into a multi
 channel polydimethylsiloxane (PDMS) microfluidic device\, enabling control
 led flow experiments and real-time monitoring of bacterial attachment usin
 g phase-shift reflectometric interference spectroscopy (PRISM). The photon
 ic chip functions both as an optical transducer and as a substrate for bac
 terial attachment. Using this platform\, we investigated the roles of surf
 ace chemistry and biochemical recognition in bacterial attachment and colo
 nization.\nSeveral silicon chemistries were examined\, including silicon o
 xide (OX)\, amine-terminated (AMINE)\, and lectin- functionalized surfaces
 . Lectins are carbohydrate-binding proteins that recognize specific glycan
  motifs on bacterial cell surfaces through their carbohydrate-recognition 
 domains\, and we hypothesized that they may enable selective bacterial cap
 ture based on differences in surface glycosylation. First\, the interactio
 ns of several lectins with Listeria innocua and Escherichia coli\, used as
  Gram-positive and Gram-negative model bacteria\, respectively\, were char
 acterized. Based on these results\, Wheat Germ Agglutinin (WGA) was select
 ed and immobilized on the microstuctured chip. Bacterial interactions with
  the different surfaces were subsequently characterized by PRISM and micro
 scopy. L. innocua exhibited substantial attachment to all tested surfaces\
 , with pronounced bacterial accumulation on the AMINE- and WGA-functionali
 zed surfaces. In contrast\, E. coli displayed a clearer preference for the
  WGA-functionalized surface. These findings show that surface chemistry mo
 dulates bacterial attachment can in a species-dependent manner. However\, 
 WGA did not provide sufficient selectivity to clearly distinguish between 
 the two bacterial species based on the PRISM response.\nOverall\, the deve
 loped platform provides a versatile framework for investigating how differ
 ent surface and environmental cues can influence bacteria–surface intera
 ction under controlled conditions. The platform can be further adapted wit
 h alternative surface chemistries\, capture probs or surface topography to
  improve bacterial differentiation and spatially selective colonization.
CATEGORIES:סמינרים
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TZID:Asia/Jerusalem
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DTSTART:20260327T030000
TZOFFSETFROM:+0200
TZOFFSETTO:+0300
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