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UID:1208@biotech.technion.ac.il
DTSTART;TZID=Asia/Jerusalem:20260722T140000
DTEND;TZID=Asia/Jerusalem:20260722T143000
DTSTAMP:20260715T132515Z
URL:https://biotech.technion.ac.il/events/%d7%a1%d7%9e%d7%99%d7%a0%d7%a8-%
 d7%a1%d7%99%d7%95%d7%9d-%d7%9e%d7%92%d7%99%d7%a1%d7%98%d7%a8-%d7%a6%d7%99%
 d7%a9%d7%99-%d7%97%d7%94-%d7%9e%d7%94%d7%9e%d7%a2%d7%91%d7%93%d7%94-%d7%a9
 %d7%9c-%d7%a4/
SUMMARY:סמינר סיום מגיסטר: צ'ישי חה\, מהמעבדה 
 של פרופ' דגנית ודנינו ופרופ' קה יין לאונג
DESCRIPTION:Research Topic: Functional Genomic Analysis of Biofilm Determin
 ants and Comparative Plasmid Genomics in Edwardsiella tarda GT163\nAbstrac
 t: ** Lecture will be given in English** (249 words)\nRapid spread of anti
 biotic resistance genes (ARGs) in the environment poses critical threats t
 o global public health. The overuse of antibiotics in aquaculture\, such a
 s bullfrog farms in Shantou\, China\, can lead to emergence and disseminat
 ion of multidrug-resistant (MDR) bacteria in the environment. Conjugation 
 is a key mechanism that drives the spread of ARGs in bacteria populations.
  Previous studies have also shown that biofilms can provide favorable envi
 ronment for conjugation among bacteria populations.\nEdwardsiella tarda st
 rain GT163\, isolated from the frog farm sediments\, is an MDR bacteria th
 at exhibits a strong biofilm-forming phenotype. Genome-wide transposon (Tn
 ) insertion mutagenesis revealed four key genes (nlpD\, wecA\, papB\, and 
 ptsI) distributed across the chromosome and plasmid that were essential fo
 r biofilm formation in E. tarda GT163. One plasmid (pGT163) from this bact
 erium is also predicted to be conjugative. Light and scanning electron mic
 rocopy approaches revealed a filamentous morphology in nlpD::Tn mutants. I
 nterestingly\, a mutation in the plasmid-born fimbrial pap gene cluster in
  the pGT163 suggests that biofilm phenotype can be disseminated by bacteri
 a conjugation. Comparative analysis of 385 Edwardsiella plasmids from the 
 NCBI database retrieved 58 plasmids that shared the incFIIrepA and 22 tra/
 trb-associated T4SS cluster. These plasmids were classified as PTU1 group 
 with three distinct sub-lineages: PTU1-1\, PTU1-2\, and PTU1-3. The pGT163
  belonged to the PTU1-3 subgroup.\nThis study defines critical genetic det
 erminants of biofilm formation in E. tarda and provides a universal classi
 fication framework for Edwardsiella plasmids that harbor these important g
 enes. These findings have broader implications for understanding biofilm f
 ormation in MDR Edwardsiella in aquaculture environments and the evolution
  of related plasmid lineages.
CATEGORIES:סמינרים
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