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UID:1045@biotech.technion.ac.il
DTSTART;TZID=Asia/Jerusalem:20210705T180000
DTEND;TZID=Asia/Jerusalem:20220202T164952
DTSTAMP:20220512T124718Z
URL:https://biotech.technion.ac.il/events/evaluation-of-encapsulated-lipas
 e-activity-within-emulsion-droplets-2/
SUMMARY:Evaluation of Encapsulated Lipase Activity within Emulsion Droplets
DESCRIPTION:Biomassis highly considered as a sustainable and economic sourc
 e for chemicals. Inparticular\, biodiesel can be produced by cellulose hyd
 rolysis to glucose\, itsfermentation to ethanol and trans-esterification w
 ith triacylglycerols fromwaste or other sources. There is current interest
  in combined enzymaticprocesses in emulsified systems due to their large s
 urface area\, localized masstransfer and ease of separation. Previous work
  described the formation of astable non-crystalline cellulose coating on e
 mulsified oil droplets. Suchcellulose-coated oil-in-water emulsion droplet
 s were shown to provide aneffective substrate for enzymatic cellulose hydr
 olysis. Ongoingresearch is aimed at utilization of cellulose-coated emulsi
 ons in simultaneoussaccharification and fermentation and combining a casca
 de of biochemicalprocesses in a “one-pot” biodiesel synthesis. The aim
  of this study is toevaluate the ability of an enzyme (Lipase\, as a model
 )\, introduced into theemulsion’s aqueous phase\, to be incorporated wit
 hin the cellulose-coateddroplets and to function effectively in hydrolysis
  of a fatty acid-esterdissolved in the emulsion core.\n\n \n\nInthis pres
 entation\, the complex structure of cellulose-coated emulsion droplets isp
 resented by cryogenic scanning-electron microscopy images. These reveal th
 einner oil core surrounded by a shell of cellulose hydrogel\, encapsulated
  by athin outer coating of amorphous cellulose. Lipase activity is evaluat
 ed by twomethods. a) hydrolysis of p-nitrophenyl dodecanoate (p-NPD) disso
 lved in n-decaneand emulsified by cellulose\, using lipase introduced into
  the aqueous medium. Activity wasmonitored colorimetrically by the release
  of p-nitrophenol.b) transesterification of methyl laurate and oleylalcoho
 l emulsified with cellulose. Activity was monitored by GC-MS followingthe 
 production of oleyl laurate. The results of bothexperiments\, and the asso
 ciated control experiments\, prove that lipaseintroduce into the aqueous m
 edium is successfully incorporated at the innerinterface within the emulsi
 fied droplets between oil and hydrogel\, and is henceactive. In addition\,
  conversion of transesterification product (oleyl laurate) is notably high
 er compared to conversion of hydrolysis bi-product (lauric acid)
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