synthetic DNA and its applications: from data storage media to high throughput biological assays

20.05.2020
14:00
Via ZOOM, ID 91168976009
Leon Anavy, Computer Science, Technion
DNA synthesis technology has greatly developed over recent years and is holding a promise to enable a leap in using natural systems for various applications. Synthetic oligonucleotide libraries (OLs) consisting of thousands of DNA oligonucleotides/sequences have become a common tool in molecular biology and synthetic biology. OLs have been used to perform systematic, unbiased investigations of gene regulation mechanisms and genetic variations by using high throughput molecular assays to simultaneously scan thousands of sequence variants. Similar OL based approaches are used to design and optimize protein engineering procedures as well as CRISPR-Cas9 based tools. Synthetic DNA is also an attractive alternative for data storage media. DNA based storage has a physical information density that is orders of magnitude better than that of magnetic media. DNA is also chemically robust. Finally - DNA, a central molecule of life, will never become obsolete. DNA can therefore potentially store data for centuries. In recent years, several studies demonstrated using OLs to store digital information on a MB scale. The talk will begin with a brief introduction of DNA synthesis technology and OLs followed by a report of two or three example projects. This will include work on DNA based storage in which we exploit inherent redundancies in DNA synthesis technologies to achieve higher logical density by using composite DNA letters. Wednesday, 20/5/2020, 14:00 – 15:00, Via zoom Meeting ID: 91168976009